Fig. 3. Activation of p38MAPK signaling pathway by uremic sera is inhibited by flavonoids and antioxidant mimetics. ECs were incubated (15 min) with sera from control donors (C), and uremic patients (U) in the absence and presence of apigenin, genistein, quercetin, ebselen, EUK134 and EUK118 (n=5). A. Bar diagrams represent the ratios of phosphorylated p38 MAPK relative to the total protein, obtained from antibody cell-based ELISA technique. B. Phospho-p38 MAPK in ECs was also assessed by Western-Blot. Images are representative of 4 different experiments. Results were quantified and represented as Mean±SD by bar diagram. # p<0.01 vs control and *p<0.05, **p<0.01 vs uremia.